Fairness datasets to evaluate machine learning models?

We are looking for the datasets that can be used for evaluating fairness models in machine learning on real data. Could you recommend a labeled dataset in which the labeling reveals some unfair...

11 November 2020 649 8 View

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04 June 2020 2,254 1 View

How to simulate ion (e.g. Zn2+ ion) in water using VASP?

I need to simulate an ion surrounded by water molecules using periodic boundary conditions using VASP. I understand, that due to waveplane basis set nature we can't set charge to particular atom....

15 January 2020 6,428 2 View

How one can measure the Flory-Huggins polymer-solvent interaction parameter?

Dear colleagues, I need to determine the crosslink density of thermosetting cyanate ester resin. Among the methods, which are presented in the literature, I chose the solvent-swelling method. For...

01 October 2019 7,302 1 View

Can i use Tween-20 instead NP-40/Triton X-100 for separation of cytoplasmic and nuclear RNA?

Hi there. I want to extract separately cytoplasmic and nuclear RNA fraction from leukemic cell line by Trizol. Most of protocols that I saw suggests using a buffer with NP-40 or Triton X-100 for...

06 September 2019 2,257 3 View

Can anyone advise a good reviews devoted for magnetic permalloy disks and their vortex state?

Hi everyone! Im going to study about magnetic permalloy disks and their vortex state. Specially, Im interesting in disks of 1,5 um in radius and 20nm of thick. But i want to get a knowledge...

25 August 2019 4,690 3 View

Has anyone used a cheap commercially available brain computer interface?

Has anyone used a cheap commercially available brain computer interface, such as "NeuroSky MindWave", or "Muse: The Brain Sensing Headband" in the learning process, in gamification? Are works on...

10 July 2019 2,863 1 View

What influence on the minimal PAO.SplitNorm parameter (SIESTA Code) ?

I am trying to perform Ab Initio molecular dynamics simulation for liquid 5-component system (TiZrNbHfTa alloy). And when I'm trying to perform it with the default value of PAO.Splitnorm (=0.15),...

22 January 2019 8,164 1 View

How to distinguish between male and female of Superlworm (Zophobas morio)?

On larva or adult stages. Do you have any ideas about automating this process?

07 July 2018 4,765 3 View

Could you please share your experience or articles about efficient methods of rearing of Mealworms (Tenebrio molitor) which for human consumption?

Do you have some thoughts about automation of process? Smth like: 'Insects as Sustainable Food Ingredients ' https://bit.ly/2Kacfdc

26 June 2018 1,457 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

GST assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...

11 February 2021 1,328 3 View

What lysis buffer is compatible with Bradford assay?

Hello! I am planning to use Bradford assay to measure protein content in PBMCs lysates. The problem is that Bradford assay doesn't seem to be compatible with Tris-based buffers or SDS. Are there...

10 February 2021 1,797 5 View

For PC-3 cell line. How long before irradiation do I have to synchronize my cells to G0/G1?

I am trying to do a clonogenic assay for PC-3 after irradiation. I have to synchronize my cells by serum starvation but there's a optimal time I have to do that before irradiation. been looking in...

09 February 2021 9,480 1 View