Hi everyone,
I am trying to get some information about caspase 1 protein homology in mammals, especially human, mouse, rabbit, rat. However, I am not orientated in this topic. Can anybody help me? :)
Thank you!
I am seeing non-motile black dots of almost equal size along the periphery of skin melanoma cell line A375. They are present in almost all the cells. They don't turn the media turbid. However...
18 January 2021 5,151 1 View
Hi everybody, I am trying to polarize and differentiate thp1 cells to M1 and M2 macrophages. There are lots of different protocols, so I've been trying different incubation times and...
02 November 2020 8,554 3 View
Hi, I have problem with my libraries, I use ClenTag Small RNA Library Preparation Kit by Trilink. The material is RNA from plasma cells. I have already done this two years ago and everythink...
25 August 2020 8,459 1 View
The major spices and ingredients to add flavour in a paper for a journal in the domain of Computer Vision and Machine Learning.
27 June 2020 7,967 10 View
What are the deciding factors to submit a paper in conference or journal.
21 June 2020 1,604 8 View
Hello Everyone !! Does anyone have any experience in publishing through Lambert Academic Publishing (LAP)? Recently I got a request mail from them regarding publication. I want to know about the...
25 April 2020 2,651 2 View
Hello :) I have the Abaqus CAE student version for Windows recently. My problem is: Abaqus does not write the .jnl and .rpy files automatically during a session as I am used to from the Linux...
30 March 2020 2,579 4 View
Hi, we are wondering about the HSV1 virus in human tissue. We want to perform in situ sequencing with this tissue, but we are not experienced with the virus-infected samples. Is it possible that...
14 October 2019 8,969 1 View
I have some 16S RNA sequence with which I want to do the phylogenetic analysis, so Initially I did BLAST against SILVA db and NACBI 16S db and went for MSA in MEGA-X using Muscle and Clustal both...
21 July 2019 9,320 3 View
I have obtained the file.log after geometry optimisation done using GAUSSIAN and I do have the file.p2n after running Ante_RED-1.5.pl then I am getting an error of not having GAMESS while I wanna...
04 April 2019 4,228 1 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...
01 March 2021 6,953 1 View
Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...
01 March 2021 9,938 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...
27 February 2021 9,233 3 View
Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...
25 February 2021 155 2 View
Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...
24 February 2021 1,825 3 View
Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...
24 February 2021 5,759 3 View
Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...
23 February 2021 7,701 2 View
Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...
23 February 2021 5,476 4 View