Contributions to Nanomaterials Special Issue "Porous Metal-Organic Framework Nanoparticles"?

20 October 2020 248 2 View

Does anybody know if there are antibodies targetting HIV TAT-peptide?

Does anybody know if there are antibodies targetting HIV TAT-peptide (just the region used for transduction: GRKKRRQRRRPQ) We wanted know if our TAT-peptide has entered the cell by Western Blott...

29 September 2019 7,692 1 View

Does anyone know of any dialysis membrane NOT made of organics (e.g. cellulose, etc.)?

I am performing some experiments and I'd need to buy a dialysis membrane (not a filtration membrane) that is not made of organic molecules. Teflon (C-F) would work, but haven't been able to find...

29 October 2018 2,906 2 View

Is there any substance that can be used to aid in organic phosphate quantification in P-NMR?

In the past I have used internal standards (IS) for quantification of organic molecules in 1H-NMR. Simply by spiking exerimental samples with a few microliters of a internal standard solution (of...

12 October 2016 10,012 13 View

Is it possible to use an LCMS column that is too long to physically fit inside the instrument slot?

I bought a new LCMS column that is very long as I require of a very good analyte separation. Just realised the column doesn't physically fit inside the LCMS instrument slot but I would swear I've...

29 July 2016 2,928 3 View

How stickiness can be reduced in dough and tortilla (wrap)?

I'm looking for functional ingredients that can be added to the formulation of tortillas (also known as wraps) and in the dough. In the same way, what process parameters affect the most this...

05 June 2016 5,888 9 View

Can I present data, as an oral presentation, that has previously been in poster format ?

I wonder whether I can give a talk in a scientific conference showing data that has previously been presented in a poster format. Thanks a lot for your time!

03 April 2016 6,240 1 View

How can I quickly detect the presence of dipeptide gly-gly in solution?

The LC-MS setup I currently dispose of wouldn't work and changing the column would require a long time so, for now, LC-MS is not an option. HPLC could be an option but the machine I dispose of is...

23 March 2016 5,676 3 View

How do I deal with quality constraints in a simulation-optimization run in Matlab?

I'm working in a simulation-optimization problem, for which I'm using Matlab. The simulation consists in solving a set of ODE's, which in turn gives information to calculate an objective function...

24 June 2015 5,587 1 View

Where can I find the exact composition of FCS?

Does anyone know where could I find a reliable list of "known" growth factors present in Fetal Calf Serum? I am a bit lost on Google. Thanks a lot!

10 November 2014 1,568 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View