Why IPNI voluntarily abbreviates some people's surnames in their standard forms?

The International Plant Name Index is known for standardising plant scientific names, including the standard forms of authors' names. I heard that one person's standard form may be chosen when...

01 January 2021 1,928 5 View

Which variables are related to transportation infrastructure?

I am conducting research on the "Impact of Transport Infrastructure on Economic Growth" Dependent Variable: GDP Growth (World Bank) Independent Variable : Railways, goods transported (million...

18 November 2020 9,014 6 View

How do I enter multiple points of I-V MOV characteristic from an external file in PSCAD?

I have a multiple points from a I-V MOV characteristic from a equipment datasheet, but PSCAD only allows to enter eleven points. I saw there is an option to enter until 100 points from an external...

28 February 2020 2,737 3 View

MOV modelling using PSCAD?

What are the main parameters that should be entered in pscad for MOV modelling? The MOV will be part of the overvoltage protection circuit of a series capacitor in a transmission line. And how do...

27 February 2020 2,055 2 View

How to create interaction plots of 2x2x2 mixed ANOVA (BWW Design) in R?

My current study looks at how Chinese and English view different advertisements that were manipulated on the length and type as...

10 May 2018 263 3 View

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31 October 2017 867 1 View

Where is the "Boca de la Raspadura" in Colombia?

24 August 2016 8,571 3 View

Can trypsin affect the cellular receptor to dengue virus infection?

The trypsin can change the VERO receptors used by dengue virus infection.

17 August 2016 1,879 3 View

How can I isolate Canine Dispar Virus (CDV) of dog plasm?

02 August 2016 2,327 3 View

How I can lyse virions?

I need a viral lysate. There are some substance to it? Something that disrupts the lipid envelope.

20 July 2016 4,734 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View