hi Ain Ali, try this  Simple procedure—just homogenize tissue sample in 1:20 (w/v) of tissue to T-PER Reagent, then centrifuge to pellet cell or tissue debris

• Mild and convenient—detergent is dialyzable for quick and easy removal; can be used with additional components (e.g., protease inhibitors, salts, reducing agents, chelating agents, etc.)

• Flexible—lysate may be used for reporter assays, protein kinase assays, immunoassays and/or protein purifications

• Compatible—the lysate is compatible with standard protein assays such as Pierce Coomassie Plus (Bradford) Protein Assay.

this is from thermo Fisher Scientific brand`s link.