Natascha Fussi

3 Questions 9 Answers 0 Followers

Questions related from Natascha Fussi

Does anyone knows some marker for the following non-classical secretion pathways?

Does anyone knows some marker for the following non-classical secretion pathways:lysosomal release; exosomal release; membrane blebbing (shedding); direct protein translocation; constitutive or...

13 December 2015 510 5 View

Does anybody know how to establish polyQ19 and polyQ80 luciferase constructs to measure autophagy?

PolyQ80-luciferase forms protein aggregates when expressed in cells that are specifically degraded by autophagy, whereas polyQ19-luciferase is soluble in the cytosol and thereby functions as a...

18 February 2014 9,524 0 View

How can we detect LC-3 II band with western blots?

I also have a problem with detecting the LC3II band on my western blot. The lC3I is not well separated from my LC3II band or the LC3II band is a smear. I use 4-20%Tris/Glycine gels from Biorad....

17 November 2013 5,212 6 View