What are the effects of longer exposure to siRNA (for KD)?

1) I knocked down a protein and collected cells 24 h and 72 h after transfection with Lipofectamine (to note, 5 h after transfection medium was replaced with medium with FBS). 2) KD efficiency...

08 December 2020 9,850 1 View

Why is hard to produce antibodies that are specific to a protein?

Some antibodies reveal nonspecific bands when a western blot is performed. Why is hard to find a good antibody for a protein? Antibodies produced using as the immunogen a synthetic peptide or a...

03 December 2020 208 3 View

Why different antibodies for the same protein stain different places?

I tried two antibodies and one show stain in the cytoplasm and the other one in the nucleus. Why? (Blue - dapi; red - mitochondria; green - protein of interest) And why is hard to find a good...

24 November 2020 6,612 7 View

Do you have experience visualising multiplex amplification curves in LightCycler 480 Sw??

We are using Cobas z480 for multiplex RT-PCR using 3 taqman probes FAM/VIX/CY5. Once the PCR finish we want to see in the amplification curve the three results for each sample at the same time....

28 October 2020 8,372 1 View

TEACHING ESL in the lockdown - participants needed?

Dear English lecturers (teachers, instructors), I am conducting research into ESL teaching to increase our understanding of how teachers have experienced special teaching conditions during the...

14 October 2020 7,223 6 View

Why does mitotracker red stains the nucleus?

I'm working with NT2 cells and I use mitrotracker red (400 nM) to stain the mitochondria. I incubate it with cell medium for 30 min, fix with 4% PFA, permeabilize with PBST (0.2%-0.5% Triton...

11 August 2020 8,076 3 View

What psychometric properties should be reported for a translated version of a questionnaire?

Dear collegues, I performed a cross-cultural study using two questionnaires (66 and 12 items). The original version of these questionnaires was used in the first country, and these tools were...

27 April 2020 4,097 9 View

USRP N210_ RF1 and RF2 dont work ?

Hi, I am working on USRP N210 and Matlab 2016R. I want a transfer a Sinusoidal wave from one port of Antenna and want to receive same wave from second Antenna at one USRP N210 (unfortinaly I...

28 January 2020 5,568 3 View

Problem in modulating miRNA expression (both over-expression and knock-down) in mouse cell lines?

Hi everybody, we are working with let-7 family and we would like to perform knock-down of the let-7 using Lin28 expression vector. In another experiment we would like to over-express let-7g from a...

20 September 2017 9,420 2 View

Can anyone help with a PCR insertion/deletion genotyping problem?

09 July 2014 4,184 3 View

In your view, which current SEM is most recommended for EBSD/TKD and EDS analysis?

I know that this is a very difficult question, as the areas of application can vary greatly. However, if you want to operate low as well as high resolution (from a few millimeters to nanometers),...

02 February 2021 4,985 4 View

What could be the reason for XRD pattern starting from very high counts/intensity at 3 degree 2 theta?

I am working on XRD analysis of the clay minerals from the sea sediments. My XRD pattern shows very high counts at the start of the XRD pattern (3 degree 2 theta) which is very unusual. The counts...

31 January 2021 4,493 9 View

Is there any special rule to find out possible room-temperature stable silicates' chemical composition, if not crystal structure itself?

Suppose one needs to find out room-temperature stable silicates chemical composition of a particular cation, suppose magnesium. Is there any rule to estimate which stoichiometric values of metal...

13 January 2021 7,385 4 View

Is there a way to identify the clay mineral based on the Si:Al ratio that has been obtained through EDAX analysis?

I have come through a few literature,were the presence of particular clay mineral has been validated through the Si:Al ratio obtained from EDAX analysis. But, I would like to understand the basic...

08 January 2021 5,982 6 View

How to start with Principal Component Analysis?

I want to carry out PCA on a set of chemical data, some of them in oxide form and some in elemental form. The oxides are in percentage and the elements are in ppm. I have understood that, the...

14 December 2020 2,201 7 View

How to identify and quantify glauconite and illite/other mica present in same sample from XRD analysis?

In glauconite bearing sand stone sample other mica group minerals(illite, muscovite etc.) are also present. I want to quantify the amount of glauconite and othermica group minerals. How, I can...

10 December 2020 5,793 19 View

How to model a graphite-bearing rock using Perplex (pseudosections)?

Dear all, I am struggling to model a rock which contains the assemblage K-white mica + Na-white mica + chlorite + quartz + graphite + rutile. I have followed step by step the tutorial available on...

22 November 2020 2,387 2 View

How much acid do I need to convert a basic solution into an acidic soluion?

Assuming that there are no chemical buffers present, can anyone confirm that if I want to convert the pH of a basic solution to the pH of an acidic solution then the amount of a monoprotic acid...

27 October 2020 9,156 7 View

Which is the best Finite Element Analysis software for Geotechnical Engineering?

Dear RG Community, As we are aware that there are almost 5-6 FEM softwares available for modelling and analysis, say, PLAXIS, FLAC, GeoSlope, Abaqus etc. related Geotechnical Engineering. I am...

13 September 2020 3,769 32 View

Why i am getting the ''no candidate found'' result whenever i search for the possible mineral peaks in Xpert Highscore software?

Currently i am working on Xpert Highscore XRD software for the estimation of clay minerals from the XRD pattern. However, whenever, i try to search minerals by using ''search and match''...

16 August 2020 3,069 4 View