Strategies for short hairpin RNA interference cassettes?

In your experience, how much does the distance between the U6 promoter and the annealed -short hairpin - oligonucleotides affect the RNAi cassette expression? How to clone a shRNA sequence...

16 January 2021 9,418 2 View

How should private health-care systems be improved to guarantee everyone's right to health?

COVID-19 pandemic has shown big social inequalities in countries with private health-care systems. Governments must pay greater attention to the right to health, that every human being must have,...

31 May 2020 7,357 23 View

I'm trying to set up a TR-FRET experiment in my lab but I'm having some troubles. Could you give some tips?

I'm working with an his-protein and I'm trying to obtain an IC50 between the protein and the biotinylated substrate and a known competitor. But the experiment is not working. I will add the...

20 April 2020 4,485 3 View

Problems with Google Scholar. Do you know how to fix it?

Dear All I am having some problems with google scholar. Some citations to my papers simply disappeared. I know that the number can be reduced when two different versions of a paper are...

01 July 2018 873 60 View

Is organic chemistry predictable?

It seems like organic chemistry consists of learning a bunch of reactions, but is there any qualitative set of rules for predicting the way a set of organic reagents will react? I know that...

30 May 2017 3,980 3 View

Are there any recognized / respected certificates for R programming or data analysis ?

Thinking about online and MOOC-type certificates for R programming and data analysis, are there any that are recognized and respected by potential graduate schools and employers? I guess if people...

11 March 2017 8,044 3 View

Hi all, what is the best method to determine the onset of bird migration and the end (day arrival), using GPS points?

There is a clear evidence in my data, but I would like to have also a statistical evidence

17 September 2016 7,140 4 View

Has anybody used Cas9 knockin mouse? If so do you know if every single cell express Cas9 constitutively?

Has anybody used Cas9 knockin mouse? If so do you know if every single cell express Cas9 constitutively?

29 September 2015 5,799 3 View

Is there a Cre line specific for the immune system?

22 June 2015 7,815 2 View

Is it possible to conduct AFM electrical measurements (such as EFM and KFM) in liquids?

I was wondering is any of the above techniques can be used for measurements in liquids. Thank you!

19 January 2015 3,178 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View