Chandra SEKHAR Amara

3 Questions 6 Answers 0 Followers

Questions related from Chandra SEKHAR Amara

How to solve this problem with Immunoprecipitation using GFP magnetic beads?

Hello all, I am encountering a weird problem in my immunoprecipitation experiments with GFP magnetic beads. I have co-transfected HEK293 cells with two genes X and Y. Gene X is tagged with Myc and...

22 January 2016 2,783 7 View

Can you help me with my co-transfection experiment in HEK293 cells?

I have Co-transfected HEK293 cells with equal amounts of two different DNA constructs. Both DNA sequences are present in pCDNA3.1+ vector background. When I performed western blots from the...

27 November 2014 5,860 2 View

Three bands appear in our RNA isolation. What could be the reason for this?

We have isolated RNA from skeletal elements dissected from a mouse pup. But when we run the RNA sample on 1% agarose gel we get three bands. RNA isolation was performed by QIAGEN RNeasy mini kit....

04 September 2014 3,763 4 View