Does anybody incubate primary cardiomyocytes of mice with palmitate or glucose ?

24 June 2016 0 10K Report

Is it suitable to use cardiomyocytes isolated from the mice to study effects of palmitate and glucose?

Badges
Science topic

More Fawzi C Alogaili's questions See All

Poured Earth Concrete ?

What are the benefits and advantages of concrete flooring when used in parking lot floors, and what are the technical requirements and general specifications that should be considered to ensure...

29 June 2024 2,171 5 View

Can you provide me with ideas about sustainable materials in construction?

I want from you, my friends, ideas and suggestions for new building materials to change people’s perception of building

27 December 2023 9,153 7 View

What does strategic importance of a nation mean in foreign policy?

16 February 2019 1,889 5 View

What is the difference between a business model analysis and the writing of a business plan?

03 February 2019 9,985 10 View

What is the difference between a business plan and a business model?

03 February 2019 605 9 View

What is the concept of inertia related to strategic arrogance?

Some organizations are driven by their continued success to adopt creative processes, which makes them push forward in the competition and thus affect the strategic arrogance that ultimately leads...

05 January 2019 4,337 6 View

Blue native gel fluorescence?

Hi, Did anyone visualize fluorescent bands for fluorescent tagged proteins on a blue native gel? Do you destain the gel after the run? If anyone has a working protocol I would appreciate...

02 December 2018 10,118 4 View

Site directed mutagenesis of 5 consecutive sites in one reaction?

Hi, I am willing to mutate 5 specific consecutive amino acids to Alanine in a certain insert using site-directed mutagenesis in one PCR reaction only. I'm scanning a certain area of my protein to...

08 January 2018 6,445 1 View

I need identification key to species of genus Nymphaea in the world?

I found new wild species of Nymphaea in Egypt. I registered it in our herbarium CAIM. The material of the new species was compared with herbaria specimens of Nymphaea in CAIM, and CAI. In...

13 April 2017 3,055 3 View

Do you explore possible Human infection from birds affected by HPAI?

I am not fully aware of all investigators.

06 March 2017 7,054 1 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...

30 July 2024 1,040 1 View

Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View

Question about Single Strain Arbuscular Mycorrhiza Fungi??

Now i am working on AM fungi single strain. What are the most effective isolation techniques for obtaining pure cultures of arbuscular mycorrhizal (AM) fungi?? Is there are best approach...

26 July 2024 6,520 1 View

Screening of EPS producer LAB?

if you have MRS broth already prepared, what is the manner you will follow for preparing hyper-sweet medium for screening of EPS producer LAB? what the concentration of saccharose or glucose you...

26 July 2024 6,606 0 View

Isolation of PLV6-Bmal-Luc plasmid?

I am currently at a stage in my research where I will be using the pLV6-Bmal-luc plasmid (Addgene #: 68833). However, I am encountering some issues with the isolation of this plasmid and am unable...

23 July 2024 5,296 0 View

What is the Principle of Cysteine-carbazole-sulfuric acid reaction with glucose?

I have been looking for the principle of Cysteine-carbazole-sulfuric acid reaction in research papers and books, but couldn't find it. This reaction is mainly used for the quantification of...

23 July 2024 3,891 0 View

The best source for amplification of ADAM17 prodomain?

hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...

21 July 2024 8,660 1 View