Does anybody have the sequence of plasmid pLEX MCS?

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How can I publish my final master project as an article for a scientif journal?

I am interested in publishing an updated version of my final master´s project in Spain and i am not sure which steps to take. I am open to suggestions.

16 July 2024 7,431 1 View

Is there a lot of difference between standard LFP batteries and the LFP used in ev cars?

Apart from the size I can´t find any information about the LFP batteries used in BYD ev, for example. Can the size affect the discharge of tha battery or other characteristics?

19 March 2024 2,229 1 View

¿Cómo se aborda metodológicamente desde el trabajo social el estigma y la discriminación asociados a las drogodependencias?

Con esta pregunta se pretende identificar los métodos y técnicas más adecuadas para abordar las razones por las que las personas drogopendientes totalmente rehabilitadas, se encuentran en...

10 March 2024 6,561 2 View

Degassing metallic-Chitosan beads for BET surface analysis, the sample doesnt resist high temperature with vaccum, recomendations?

Hello community, i have trouble degassing Chitosan beads for BET surface analysis, the sample doesnt resist high temperature with vaccum, any recomendations, previous experience analyzing BET on...

16 January 2024 3,430 1 View

¿Why increasing the effective surface area decreases the current density in microelectrodes?

To characterize the electrocatalytic activity of the microelectrodes, the effective surface area was determined using the equation proposed for cylindrical microelectrodes by (Szabo, 1987). But, I...

14 January 2024 8,116 3 View

Gaussian NMR calculation error: Bad dimensions in Pcck?

Hello gaussian users I'm trying to calculate a NMR spectrum of a macrocyclic compound, I was able to optimize the molecule but not able to get the spin-spin couplings. The program shows this...

07 January 2024 3,223 0 View

Relationship between the Austrian theory of economic cycles and the bullwhip effect?

Is there any relationship between the Austrian theory of business cycles and the bullwhip effect in the supply chain?

05 January 2024 1,754 1 View

Why is the pressure inside a vertical half ton tank higher than the vapor pressure of the chemical inside it at room temperature?

The chemical is ethyl chloride also known as chloroethane and the material of the tank is carbon steel. We connect these tanks in series and push the chemical from tank to tank in our transfer...

04 December 2023 6,991 1 View

Bad quality results in Sanger sequencing?

Hello everyone. I am struggling to get good quality results on my Sanger sequencing reactions. I don't know the specific problem that I am facing. In a single order, most of the chromatograms...

03 November 2023 4,035 0 View

Hello community! For those doing experiments with retinal in plants, Do you grow your plants in darkness during the retinal treatment?

Since retinal is a photosensitive compound, I wonder whether you perform your experiments in darkness or do you allow certain amount of light. I've read some experiments for root development and...

15 October 2023 4,863 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Issues with Protein Expression in Transfected cells?

Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...

30 July 2024 6,648 1 View

Does anyone have experience electroporating SKOV3 cells with a large plasmid using Neon transfection?

I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?

29 July 2024 3,229 1 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

What is the best way to drop a plasmid?

Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...

25 July 2024 2,532 3 View

Isolation of PLV6-Bmal-Luc plasmid?

I am currently at a stage in my research where I will be using the pLV6-Bmal-luc plasmid (Addgene #: 68833). However, I am encountering some issues with the isolation of this plasmid and am unable...

23 July 2024 5,296 0 View