Does anybody have the sequence of plasmid pLEX MCS?

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How Can I solve this mistake in autodock?

ERROR ********************************************* Traceback (most recent call last): File "C:\Program Files (x86)\MGLTools-1.5.6\lib\site-packages\ViewerFramework\VF.py", line 898, in tryto...

15 November 2020 1,479 2 View

What criteria do you have in mind while characterizing a scientific theory?

I hesitate a lot before raising the question just mentioned. As RG is mainly a scientific forum, it is natural that the concept of theory appears in many posts from many posters. But what are...

25 October 2020 7,643 34 View

What is the (average) DNA yield (ng) from one single nematode?

Do you know where I can found published information about DNA quantity (in ng) from one single nematode? Any publications/books to suggest me? I Know it seems a simple and basic information, but...

31 March 2020 6,710 1 View

How could I use Solvate gromacs command for solvate on Z axis of a monoclinic box, whitout living water molecules outside?

Currently I use Gromacs to perform molecular dynamics of tobermorite 11A, but it turns out that this structure has a monoclinic box, I need to solvate my structure only above in the z axis, but I...

26 March 2020 1,208 3 View

Is there a principal responsible for water management?

The water crisis is a situation that affects us all, however: Is there a principal responsible for water management?

16 January 2020 8,671 6 View

IHC perfused brain tissue disintegrating following TBS wash on Super Frost Slide?

Hi all, I am using IHC to look at various neuronal markers in zebra finch brain tissue. Finch brains are perfused with PBS and then 4% PFA and placed in a sucrose solution overnight. I then...

01 January 2020 8,400 3 View

What is the expiration of the RPMI 1640 medium once the FBS and antibiotics has been added?

I had a medium stored and sealed for 2 months that presents an intense pink color, I put it in the CO2 incubator but it does not recover the light pink color. I will make a new medium but, has...

10 November 2019 1,632 2 View

What do you think is the most commonly used method for systematic literature review?

In my case I consider that it is the PRISMA, it has many advantages among them it allows to improve the report of the systematic reviews and of the meta-analyses. They adapt to tools such as StArt...

09 October 2019 2,769 4 View

Any source for real time control in PYNQ boards?

Real time control in parallel robots is a requisite. Python has popularity for scientific computation thanks to Scipy and Numpy libraries. Hardware implementation of control routines reduce...

21 July 2019 3,783 1 View

How to calculate the empirical power to compare the performance of two FANOVA tests in functional data?

Hello, do any of you know how to calculate the empirical power of a FANOVA test in functional data analysis? or if anyone knows a script to calculate it.

16 January 2019 10,042 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View