Shridhar Kinkar

7 Questions 55 Answers 0 Followers

Questions related from Shridhar Kinkar

Is it possible for an E. coli cell to have different morphology during growth?

I have been growing E. coli host in SM6 medium for fermentation. During the run, I did microscopy and found long filamentous rods, which are Gram negative. I have started searching literature for...

05 May 2019 4,431 9 View

Which carbon source is preferred by E. coli?

I need to perform an experiment in which I have to put both glycerol and lactose. My doubt is which of these carbon sources will be consumed first by E. coli cells? I assume it is lactose, but...

04 April 2019 3,972 5 View

Why the RCB (Research Cell Bank) is unstable?

Presently I'm working on a host-vector combination, where the E. coli host is K-host and the vector is p15A ori-based vector with tetracycline as selection marker. The problem I'm facing is the...

07 July 2018 577 0 View

Is tetracycline light sensitive? Esp. when used in bacteriological media, is it prone to photo-degradation?

I want to use tetracycline as selection marker for my next experiments. I was advised to perform the experiments in dark. I want to know if this precaution is really required. I haven't found any...

02 February 2018 4,449 7 View

Need to know if higher IPTG concentrations make E. coli expression host undergo a metabolic stress?

Hi, I have been titrating best IPTG dose for highest expression of my GOI. However, I found that after a certain concentration (2.5mM), post-induction OD600 of culture shows lesser growth and as a...

01 January 2018 3,641 5 View

What is the best way to purify tagless and soluble TNFa?

I expressed TNFa in E. coli BL21 (DE3) host and got satisfactory expression. The protein is tagless. My question is how do I purify it? Which chromatography techniques can I use and what should be...

09 September 2014 7,593 18 View

Which is the most suitable E.coli host for expressing TNF-alpha?

I'm trying to express the protein in E. coli, but not sure which host to choose. BL21(DE3) doesn't express it very efficiently. Expression conditions are 37C/0.3mM IPTG/4h.

07 July 2014 767 7 View