I'm wondering if anybody has used them for gene fragment synthesis and combinatorial libraries. I've heard some bad things about them promising things they couldn't deliver and very slow turn-around.
Hi hive, We are trying to use ccdb as a counter selection marker. We are using the cassette from this paper, which should be highly expressed: A new method to customize protein expression vectors...
22 June 2020 5,084 5 View
Dear colleagues, I am trying to determine the appropriate statistical test for my problem. I have read several discussions on the topic here, however was unable to extract an answer, and so I...
03 May 2020 6,281 10 View
I am performing DFT-calculations (gamess-us) on a reaction: ground state 1 -> transitition state -> ground state 2. (in short: gs1 -> ts -> gs2.) ts is only 0.3 kcal/mol higher in...
16 February 2020 2,261 1 View
Machine learning has proven to be very successfull in many disciplines and the general development is surely furthered by openly accesible algorithms and datasets for the whole community to work...
23 October 2019 6,941 19 View
Can there exist societysystem including projections of pictures and video frames out of thought into the physical reality? Is this possible for nature of human beeings or has this to be technical...
30 May 2019 2,426 5 View
Hi! I can not find a list of growth factors that secrete neuronal stem cells. Usually everyone writes about neurotrophins. But I think there should be other factors ( fgf, egf, igf, vegf)?
12 November 2018 9,084 2 View
I would like to calculate the optimal sample size (based on power calculations) for the probit model and a binary covariate.I have run a pilot study for a randomized control trial. I am interested...
03 September 2017 5,895 3 View
Apart from plasma activation, PDMS can be made hydrophilic by treating it with Piranha solution (and maybe KOH in an elaborate approach). I found two papers reporting this effect. Interestingly,...
18 April 2017 1,857 4 View
Additionally details of measurement for the distance would be appreciated, but is not mandatory.
01 November 2012 2,944 2 View
Please have a look on my publications Georg Oesterdiekhoff For example Child and ancient man American Journal of Psychology 2016
01 January 1970 8,102 5 View
Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...
01 March 2021 8,766 3 View
To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...
23 February 2021 7,326 5 View
Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...
18 February 2021 2,769 4 View
Hello We are working on a PCR product with an approximate size between 5000 and 7000 bp, we want to use it as a positive internal control, since we have sequenced it and it corresponds to the...
17 February 2021 2,896 3 View
Can anyone recommend some any software/models available that can predict substance reactivities and the reaction yield under a specific set of reaction conditions process and proposing pathways to...
17 February 2021 2,383 3 View
I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...
17 February 2021 8,986 2 View
Dear colleagues. Fragments of this species are very rare from a terrestrial Lower Cretaceous locality from Germany. Up to now from this locality sauropods could not be proved with certainty. Other...
14 February 2021 2,310 1 View
I am doing research on the synthesis of paracetamol. I am preparing acetic anhydride solution with a few drops a sulfuric acid and wish to measure the pH of this solution. However my pH meter...
14 February 2021 8,699 3 View
I have chemically synthesized silver nanoparticle to combat microbes on wound site. I have used many methods of synthesis, but I found minimal field of silver NP product.I am getting whitish...
11 February 2021 4,964 13 View
I want to create .abs file ( absorption) for simulating solar cell in SCAPS. Can anyone pls provide me information regarding the libraries (websites) where from i can get the absorption data of...
10 February 2021 6,034 7 View