I'm wondering if anybody has used them for gene fragment synthesis and combinatorial libraries. I've heard some bad things about them promising things they couldn't deliver and very slow turn-around.
Dear Peptide Chemists, in SPPS we forgot to deprotect the Fmoc protection group of the N-term on a complicated enough product. It's not cleaved from the resin with standard TFA protocol, washed...
27 February 2024 3,700 1 View
In the recent (quite controversial) paper about "Assembly theory" (https://www.nature.com/articles/s41586-023-06600-9#data-availability) the authors propose a way how to compute the complexity...
21 December 2023 1,753 0 View
What effects does the zero-interest and negative-interest policy of the central banks have on the willingness to take risks of private investors in venture capital and private equity firms,...
30 January 2022 1,124 6 View
Shadow banks, which played a key role in triggering the recent global financial crisis, and venture capital companies that followed, have partly displaced traditional banks from corporate...
30 January 2022 6,333 8 View
I'm not yet aware of an existing (open) implementation that combines DREAMPlace with a commercial tooling for the Routing and Signoff. Dear crowd: Do you know anything about that? Link to...
03 November 2021 631 0 View
Can you explain a list of information and matter that is spread from the outer limit to the inner point in a polarcoordinate system - the outher way around is a norlmal incrementing model while...
23 June 2020 1,633 0 View
Hi hive, We are trying to use ccdb as a counter selection marker. We are using the cassette from this paper, which should be highly expressed: A new method to customize protein expression vectors...
22 June 2020 5,230 5 View
Dear colleagues, I am trying to determine the appropriate statistical test for my problem. I have read several discussions on the topic here, however was unable to extract an answer, and so I...
03 May 2020 6,448 10 View
I am performing DFT-calculations (gamess-us) on a reaction: ground state 1 -> transitition state -> ground state 2. (in short: gs1 -> ts -> gs2.) ts is only 0.3 kcal/mol higher in...
16 February 2020 2,414 1 View
Machine learning has proven to be very successfull in many disciplines and the general development is surely furthered by openly accesible algorithms and datasets for the whole community to work...
23 October 2019 7,070 19 View
We assume this to be true. We also assume that the vacuum bomb is the latest version of explosives with an explosive power of a few to ten kg of TNT equivalent. It has the unique characteristic of...
04 August 2024 4,534 1 View
Thanks
22 July 2024 1,143 3 View
Suggest the best and shortest route for the chemical synthesis and and green synthesis of magnetite(Fe3O4) The chemical synthesis should contain the least dangerous chemicals along with full...
21 July 2024 2,476 2 View
We are in need of primers for a PCR protocol for chicken genome. We are looking at the COI barcode, 648-bp fragment of the 5' end of COI. Specifically Primers FalcFA, BirdR1, CO1_ExtF,...
16 July 2024 118 2 View
Organic Synthesis. My student has been trying iodination of arenes (our goal is to iodinate p-phenylenediamine) following these literature procedures: 1. Synthesis 2004, No. 11, 1869-1873 using...
16 July 2024 6,630 1 View
I make polycrystalline diamond with tape casting followed by HPHT sintering. When i sintered the samples up till May, the sintering was good. When i sintered the samples in June and July at the...
16 July 2024 3,212 5 View
I am doing qPCR but my HPRT1 Ct is not coming at the same point. I see a 2 cycle spread in Ct. I have checked my RNA quality A260/280, 230/280 values, did ethidium bromide gel. I used superscript...
04 July 2024 5,605 3 View
I'm currently working on gel purification in order to get DNA fragment for T4 ligation. But I have been able to take only 8.5ng/μl as a highest concentration of DNA fragment. And could not get...
01 July 2024 4,912 9 View
Please, can the fragmentation obtained from the mass spectrometry be predicted more easily using any free software? Kindly recommend it, thanks.
01 July 2024 5,327 6 View
I want to isolate RNA from bacillus subtilis using the QuickRNA Fungal Bacterial Miniprep Kit from Zymo Research and further reverse transcriptase to cDNA using the ReverTra Ace qPCR RT Master Mix...
27 June 2024 2,951 5 View