We want to transfer embryos produced in vitro to synchronized recipients at day 4.
You can transfer morula just like blastocysts into the uterus. If you want to put them in the oviduct, you will need precise ultrasound techniques and specialized equipment. What is your aim?
We want to flush again at day 9 - with in vitro we have to many artefacts, which is why we want to transfer.
We already transferred at day 4 and flushed on day 9, but we were not able to retrieve anything - now we will try day 5.
What are you studying in the embryos? I assume you are interested in what happens around the time of hatching or thereafter?
Yes, we want to study second lineage differentiation, which happens by day 9.
I think the great challenge in tranfer at day 4 is to synchronize the recipient to the embryo - if the uterus is not "ready" for the embryo, because ovulation occured a little later, the embryo cannot survive. Now we will transfer at day 5, day 4 is just too tight a timeframe (oviduct passage ends at 3.5).
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