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We want to transfer embryos produced in vitro to synchronized recipients at day 4.
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I'm using sc-7244 (1:50) from santa cruz in bovine E9 embryos after 2% PFA fixation. I permeabilize with 0.5% TritonX (1h, RT) and block with 0.1% TritonX (20 min), all solutions contain 10%...
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