Does anybody have experience with GATA6 staining in embryos?

I'm using sc-7244 (1:50) from santa cruz in bovine E9 embryos after 2% PFA fixation. I permeabilize with 0.5% TritonX (1h, RT) and block with 0.1% TritonX (20 min), all solutions contain 10%...

21 January 2016 5,342 2 View