I have many miRNAs to analyze using Taqman probes and I would like to save some time/money during the cDNA synthesis by pooling some primers in the same reaction. The standard protocol recommends one primer by reaction, but I know that some people have successfully performed this pooling. Does anybody have an already established functional protocol for this?
Hi, I rememred to have the same problems and I used this protocol (maybe there is an update versions now).
Hope you can find this useful!
Hi, I rememred to have the same problems and I used this protocol (maybe there is an update versions now).
Hope you can find this useful!
Hi, I usually work with miRNAs and always use the protocol of Valeria. It is important that they have a final concentration RTprimers
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