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13 June 2017 7,057 4 View

Does anybody have experience with expressing proteins in the surface of mammalian cells (i.e CHO or HEK cells)?

Hi. I'm using the pDisplay vector from Invitrogen to express in the membrane surface a cytosolic domain of a protein. The problem I have is that the protein is not displaying in the surface, I...

04 May 2015 9,229 3 View

Does anybody have experience with expressing proteins in the surface of mammalian cells (i.e CHO or HEK cells)?

Hi. I'm using the pDisplay vector from Invitrogen to express in the membrane surface a cytosolic domain of a protein. The problem I have is that the protein is not displaying in the surface, I...

03 May 2015 9,729 3 View

What is the best buffer to obtain a circular dichroism spectrum of a protein?

19 February 2015 9,492 6 View

Can someone recommend a fluorescence or absorbance protocol to evaluate Caco-2 monolayer integrity for transport experiments (apart from TEER )?

I am culturing Caco-2 cells on filter supports and I have been evaluating monolayer integrity using the Endohm tissue resistance measurement. I would also like to use a colorimetric or a...

01 February 2015 5,732 3 View

What is the best buffer to obtain a circular dichroism spectrum of a protein?

01 February 2015 8,966 7 View

Can anyone help me to use wP97X-D compared with MP2?

I have done a couple of calculations with this dispersion corrections and range separated functionals. The complex under study is Cinnamic---DMSO from XRD studies, where the ethylene group is 3.4...

11 January 2015 7,090 3 View

Are you interested in participating in a conference on East-West intercultural relations in NY in March?

Dear Colleagues, The title of our yearly conference is "Intercultural Dialogues and Crossings among the East, the Americas, and the Iberian Peninsula." The keynote speaker will be the Mexican...

10 November 2014 730 0 View

Is this kind of calculation been done (in aromatic)pi---n(S)

Prediction of distances and potential are common aims in paramagnetic compounds. But what about paramagnetic through space interactions? I'm interested on induced spin densities and its mechanisms.

17 September 2014 7,852 1 View

Does anybody have measure fluorescence in a plate reader?

08 September 2014 420 4 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View