Hello Humaira

You can isolate the inoculum of this bacteria form plant debris (tomato or pepper) or from seeds that collected locally from fruits of these plants (infected fruits). I attached the following references, possibly useful for suitable growth media and for inoculation techniques.

Good luck

Thank you for your kind suggestions. However, I am unable to find any infected fruit. In that case, what am I suppose to do?? And after isolation how do we characterize that it is the same strain and not some other strain. Please if you can help.

You welcome Humaira 

If you find difficulties to get the isolate by yourself through isolation from soil, fruits and plant debris, you have to get it from other departments that working with project including this bacteria (Xanthomonas campestris pv. vesicatoria).

Also, for studying the characteristics of this bacteria , you have to do  physiological and biochemical tests on the isolated bacteria using the methods described by many references. Otherwise, you can use molecular techniques to confirm the identification. Good luck

under www.dsmz.de you can obtain the isolate. Best regards. Werner Solbach

Xanthomonas vesicatoria

DSM-22252

Xanthomonas vesicatoria

ATCC 35937, CFBP 2537, ICMP 63, LMG 911, NCPPB 422

Thank you so much Werner Solbach. I will look up.

Thank you Jawad Abood Al-Janabi  for your help