Why does a Nested PCR protocol amplify samples and not amplify positive control?

I have this problem. Today I used a new positive control of an isolated NC-1 strain in cell culture and the problem persists. I performed a serial dilution test to test the sensitivity of the...

08 December 2020 6,252 2 View

How to avoid the formation of traces and dimers in DNA gel electrophoresis?

07 December 2020 3,052 3 View

Is the pipetting order of the positive and negative controls in the PCR important to avoid in a possible contamination?

I have been watching bands form on my negative controls. I have already changed all reagents, optimized the hygiene of materials and the environment and increased care in pipetting.

07 December 2020 9,090 3 View

How to standardize and obtain a good PCR reaction with samples with a low and variable amount of DNA?

I work with the detection of N. caninum DNA in milk samples in milk samples. The DNA concentrations in my samples range between 2-90 ng / ul. I would like to establish and standardize a final...

01 December 2020 8,792 3 View

Measure of intracellular lactate?

Hi! I will try to measure intracellular lactate through lysing the cells in 20 mM Tris-HCl, 150 mM NaCl and 1% NP40. I'm going to use a clinical kit, which is constituted of Pipes buffer, pH 6.8....

30 January 2020 5,211 2 View

Dissolved oxygena DYE for cell culture?

Hi, I am looking for a dye to monitor O2 changes in cell culture media. I would like to have it colorimetric and not fluorescent but since I am not finding anything I could to fluorescent too! I...

23 October 2018 5,895 2 View

How do I determine the sample size to evaluate the performance of an image segmentation algorithm?

I have three image segmentation techniques that I have developed. I wish to compare the performance of the segmentation techniques using measures such as BDE(boundary displacement error),...

11 October 2018 2,021 5 View

Most appropriate Feature Selection to find indicators that are most correlated to the number of one event?

I have two databases. One of them contains the number of this event per year. The other one contains a lot of indicators and its values per year. I want to find those indicators that are most...

20 March 2018 3,610 4 View

Which is the best way to treat ticks and fleas for morphological identification before performing molecular investigations?

Hello all, I am trying to attempt morphological identification of ticks and fleas collected in Wales on wild small rodents. My samples have been frozen and after the morphological identification I...

10 October 2017 7,374 4 View

How can i stain dopaminergic neurons in a rodent?

im working in resource limited lab in uganda but l was wondering whether there is a cost effective technique l would use to stain dopaminergic neurons and get valid results? thank you so much in...

23 May 2016 4,994 2 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

How COVID-19 has affected mental health?

The pandemic has led to depression and anxiety and its negative effects shouldn't be ignored. However, it has also led some people to become more resilient! What do you think? Thank you.

21 February 2021 4,733 19 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Can medicinal plants really play an inherent rule in the COVID-19 tackle?

Numerous scientific reports cited plant extracts with potential antiviral activity and even anti-COVID-19? Why these findings are neglected? I remember very well the warning released by the WHO to...

17 February 2021 2,141 9 View

How can I interpret a ratio variable?

Dear colleagues, I am running a regression in which I have the following regression FoodExp = 67.04 DepRatio +.....e My interest is in the interpretation of the coefficient on the dependency...

16 February 2021 2,058 2 View

Despite single-cell RNA, how can I characterize a new cell subtype/phenotype?

A new cell subtype was found out only during regeneration phases after a cuprizone-induced demyelination model. How can I characterize this cell? It is important to highlight that was already...

13 February 2021 1,245 1 View

Do you know of an informal caregiver of a person who has recently received a bone marrow transplant?

We are conducting a pilot study of an intervention for informal caregivers of persons who have recently received a bone marrow transplant in the US. If you know of anyone who might be interested,...

13 February 2021 9,174 1 View

Is anyone else having a similar situation?

I'm looking for the genetic sequence for the TAR region of HIV. If I put Transactivator Response Region, and HIV, and "genetic sequence: into a browser...is it not unreasonable to expect to be...

10 February 2021 658 5 View