I want to know, is there any guadlines for chemicals analysis?
For example an Acetonitrile HPLC Gradient Grade product includes this specification,
Acidity ≤ 0.0002 meq/g
Alkalinity ≤ 0.0002 meq/g
Gradient grade (at 210 nm) ≤ 1.0 mAU
Gradient grade (at 254 nm) ≤ 0.5 mAU
Fluorescence (as quinine at 254 nm) ≤ 1.0 ppb
Fluorescence (as quinine at 365 nm) ≤ 0.5 ppb
Transmission (at 193 nm) ≥ 60 %
Transmission (at 195 nm) ≥ 80 %
Transmission (from 230 nm) ≥ 98 %
What must be the limit for each analysis ?
The limit is set by the chemist who is using the reverse phase solvent. Their criteria is whether it interferes with a particular assay or method.
Mrs/Miss. Dagdelen,
Pleas pay attention to:
http://www.merckmillipore.com/DE/de/products/analytics-sample-prep/spectroscopy/solvents-for-spectroscopy-uvasol/Skyb.qB.BD0AAAE_ahV3.Lxj,nav?ReferrerURL=https%3A%2F%2Fwww.google.de%2F
I add some interesting information in the attached file. There is also a suitability test for LC-MS purity for acetonitrile and methanol based on reserpine signal. No signal of background must be higher than that of the molecular peak of reserpine (609 amu) at a concentration of 100 ppb, in a range from 100 to 2000 amu.
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