I am setting up experiment to isolate RNA from human blood cell and use further for gene expression study.
Yes, you can try with PureLink® Total RNA Blood Kit of Invitrogen. I hope it will works well to solve your purpose.
The ATCC media preparation mentions trypticase and I am not able to find trypticase from any company like HiMedia, Merck or Sigma
26 October 2020 1,822 6 View
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04 July 2020 9,111 0 View
I am new to ns3 and I want to know if it is possible to simulate blockchain network on NS3 and if yes, can anyone share some links to tutorials that would be helpful for this. It would be helpful...
08 March 2020 7,384 1 View
I want to use Concentrating solar cell developed by Spectro lab, USA and wish sole guidelines for the same. Is it possible to store the power generated by these cells be stored by any type of...
06 January 2020 8,313 3 View
I have a purified plasmid having the insert of 655 bp. when i am pcr amplifying it with gene specific primer ideally i should get single amplified band of size 655 bp but after running it on gel i...
26 July 2019 1,762 7 View
I have to simulate multipath fading channels and add randomness to it.
21 September 2018 6,377 2 View
I successfully amplified my samples using Primer set 1 but when I'm amplifying the same samples using different Primer set, the genomic DNA is still stuck in the Wells and only ladder resolves. My...
09 July 2018 9,186 13 View
Please suggest me how can I better place the variables in the following choice experiment. CE1. Please look at the following scenario and select the level of self-driving car you would consider...
03 April 2018 1,864 2 View
Hi, What could be the reasons that a film prepared by casting a film forming solution on petri plate gets bubbles into it? How can it be prevented? Thanks, Ashish
08 February 2018 3,804 4 View
please suggest me procedure
03 November 2017 6,769 5 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...
03 March 2021 687 1 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...
02 March 2021 6,036 1 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...
02 March 2021 2,246 2 View
Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...
01 March 2021 8,235 2 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View