Does any one have papers or information about dyeing wool with alizarin yellow?

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In the age of Iconic architecture and global trends, can we still design for people? would iconic architecture enhance identities or destroy them?

In historical cities , would iconic buildings add to the character of the cities or change it ? How would we maintain their character , and yet have expressive new buildings ?

03 December 2020 2,740 18 View

How can we measure absorbance of solution contain nanoparticles by UV light if the solution was colored? Is this possible?

I have greenish-blue solution (from CuSO4 and bacterial supernatent), and I wont to make sure (by UV) if there are nanoparticles in this solution. How can I know, and what is expected range of...

13 July 2020 5,350 6 View

How to train convolutional neural network for enhancing images?

I am new to DL. I am working in Matlab. I want to train the CNN on a set of images to enhance its brightness/contrast. I want to know how can we train and test the CNN for a set of images. any...

27 May 2020 2,459 6 View

Why is my alpha tubulin looks like that?

Hi everyone, I have problems with western blotting. Some times I will get clear bands and other times I will get nothing even though I ran the same sample and used the same blocking, and...

26 March 2019 2,334 4 View

Can anyone help me understanding how to use DDSCAT 7.3 ?

I have downloaded DDSCAT 7.3 and also read user manual. However, as i am from experimental background i am facing a lots of difficulty in learning DDSCAT. Can anyone suggest me some basic...

20 February 2019 1,879 3 View

Anyone worked on Quantum Image Processing?

I'd like to do a quantum image processing with using QuTip python package. Did anyone try to do so?

25 January 2018 1,795 1 View

Which program can be used to visualize interaction of protein surface with solvant molecules?

I need an easy free program for visualization of protein solvent interaction. which software do you recommend for this?

28 March 2016 1,291 3 View

How can I concentrate extracted nuclic acid after extraction?

22 March 2016 393 5 View

MD simulation using gromacs, using the command gmx solvate?

I am simulating a protein in a mixture of water and pyridine as a cosolvent I generated a box of water+py using packmol software can someone tell me what exactly the steps I need to do solvate on...

14 February 2016 2,510 7 View

How can I extract and purified water soluble vitamins?

When i work with water soluble vitamins produced by microorganisms i measure it in crude extract. Is any one know how i can extract them by easy method?

02 June 2015 6,287 2 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

Why stop the reaction in ice for the fluorescent probe FDA (fluorescein diacetate)?

Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...

08 February 2021 3,366 3 View

What is the possible cause of MSCs low growing?

Hello Everyone, We are inexperienced in culture of MSCs trying to grow a sample from BM of a mare. It is the second time that after preparation of new medium (DMEM-F12 with high glucose - 10% Hy...

07 February 2021 9,989 7 View

Why do cells die after adding MTT dye?

I was doing MTT assay, and in the last day before adding the MTT; the cells looked good (or so I think), but after aspiration of the drug and addition of MTT dye I noticed that most cells seemed...

07 February 2021 3,846 4 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View