I plain to use cre-ert2 system to delete the loxp-gene in pup mice at P1, injection to so small pup directly have some difficlit to me, so I plan to inject to mum mice, does any advice to this?like does, frequence,duration? does the lactating mice is more sensitive to tomoxifen?
thank you for your help!
Hi,
I found an article containing tamoxifen administration in lactating mice. The authors observed that the mothers tended to abandon their offspring after 5 days of consecutive injections. The article is entitled "Inducible Gene Manipulations in Serotonergic Neurons", by Weber et al.
Other articles also describe perinatal administration of tamoxifen. For your reference, one is entitled "Perinatal or adult Nf1 inactivation using tamoxifen-inducible PlpCre each cause neurofibroma formation" (by Mayes et al), and the other one is "Inducible Site-Specific Recombination in Neural Stem/Progenitor Cells" (by Chen et al).
Hope this helps!
Kindes regards
Hi,
I found an article containing tamoxifen administration in lactating mice. The authors observed that the mothers tended to abandon their offspring after 5 days of consecutive injections. The article is entitled "Inducible Gene Manipulations in Serotonergic Neurons", by Weber et al.
Other articles also describe perinatal administration of tamoxifen. For your reference, one is entitled "Perinatal or adult Nf1 inactivation using tamoxifen-inducible PlpCre each cause neurofibroma formation" (by Mayes et al), and the other one is "Inducible Site-Specific Recombination in Neural Stem/Progenitor Cells" (by Chen et al).
Hope this helps!
Kindes regards
I can't give you much advise on injecting lactating mice, but if you are planning to induce CreERT2 mediated gene deletion in newborn pups, there is an excellent methods paper that guides you through all the steps (see attached). The described methods worked very well in my hands.
Kind regards,
Martin
Article Inducible gene targeting in the neonatal vasculature and ana...