Does any have any experience on RNA extraction from Formaldehyde-fixed tissues using QIAGEN (Cat# 73504) ?

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Nouh Saad

Dear Hao Feng, I would recommend you to extract the RNA from fresh tissues since formaldehyde breaks down the RNA from the starting of the fixation process. And the older the FFPE, the lesser RNA quality you get.

Hao Feng

Hello Nouh,

Many thanks for your reply.

Yes, you are absolutely right, to extract RNA from fresh tissues is much better than that in the PFA-fixed ones. But I have to use this approach to collect RNA in my case for downstream assay.

Thanks a lot !

Best wishes,

Hao

Emmanuel Edwar Siddig

Totally agree with Dr. Nouh Saad