Any reference on how to graft ligand protected metal nanocluster on oxide support (TiO2, SiO2)?
Hello everyone, I am currently working on a protein that is 10x his-tagged and positively charged (predicted pI=10.16). But when I tried to use Ni-NTA column to purify the protein, it's not...
24 July 2024 7,293 4 View
Hello, I am a master's student currently conducting lentivirus transduction experiments in Jurkat cells. The virus is not effectively entering the Jurkat cells, so I am planning to use an...
21 July 2024 4,676 4 View
My negative control cells had some damage, they are neoplastic cells and my anaplastic cell had even more damage in the negative control. Ok, but my question is, is there much difference between...
27 May 2024 7,529 2 View
I tested DNA structure (5uM dissolved in 50 mM Tris) using JASCO-1500 but found no peak at wavelength 220-350. The results look like a wave shape throughout the wavelength...
14 April 2024 4,003 2 View
Hi: I am performing immunofluorescence staining on 100 um 4%PFA fixed pancreas sections using our self-made polyclonal primary antibody. Tissue was gradient dehydrated in methanol diluted in 0.2%...
28 January 2024 867 3 View
We used mouse cell line HEI-OC1 and checked OCTN1 expression by WB, then we did IP cell lysate with anti OCTN1 antibody and IB with OCTN1 antibody, surprising! The band is way above IB with WB (30...
04 January 2024 6,926 0 View
17th refers to the experimental parameter code Material is mesoporous silica nanoparticles (MSN-NH2). Use deionized water as dispersant and ultrasonic vibration. The theoretical concentration is...
02 January 2024 4,160 3 View
In our study, each participant rated several organizations randomly selected from a big pool on some attributes (denoted as) x1, x2, and x3 and an outcome y, and each organization has multiple...
14 November 2023 2,756 5 View
Hello researchers, I am a student at the SOMT, university of Physical Therapy(The Netherlands) and I am studying for a master degree in Geriatric Physical therapy. Currently I am working on a...
03 October 2023 4,444 1 View
Hello, I am trying to set up my own website which will allow users to log in and open 2 seperate doors on my property. I have already made a website with the log in form and there is a system...
18 September 2023 5,682 1 View
I need to do some experiment with it in liquid form.
07 August 2024 613 3 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
Dear Siesta Users, I installed Siesta 5.0.1 and I want to use Grimme's D3 correction. According to the 5.0.1 manual, I could use a few parameters to include the D3 correction in the...
27 July 2024 5,748 0 View
Thanks
22 July 2024 1,143 3 View
Suggest the best and shortest route for the chemical synthesis and and green synthesis of magnetite(Fe3O4) The chemical synthesis should contain the least dangerous chemicals along with full...
21 July 2024 2,476 2 View
Dear Researchers, My question is associated with the "MDCI" method in Orca. Please let me clarify my question using a simple example: Suppose we are going to perform CBS extrapolation using "!...
21 July 2024 1,632 0 View
I want to calculate the spin texture for the electronic band structure obtained from SOC calculation. Is there any way for calculation of spin texture using quantum espresso??
19 July 2024 8,571 1 View
Image Processing Algorithms, Quantum Computing.
17 July 2024 7,958 2 View
In recent years, quantum computing has emerged as a groundbreaking technology with the potential to revolutionize various fields, including artificial intelligence (AI). AI has already made...
17 July 2024 1,398 3 View