I need to design primers for cloning a gene into pCAMBIA vector. The plan is that the first AUG of the gene of interest must be at +1 of CaMV35 S promoter. I am thinking if any restriction site downstream of 35s promoter can be a candidate?
another question is that whether only one restriction enzyme or two is preferable? I need to add them at the end of the primers as you know.
Best