I have a mouse primary antibody and I would like to use the same in the rat tissue for IHC purpose. Does that really work?
Thank you
Harsha
Hy,
of course you can use primary mouse antibodiesand detect them with secondary anti-mouse in rat tissue.
You can do also the inverse way and use rat primary antibodies in mice.
Good luck
Stefan
Hi Stefan,
Thank you for your answer
Unfortunately, secondary antibodies against murine primary antibodies cross-react with rat antibodies. This results in a nonspecific IHC reaction
Hi Igor Ivanovich Babichenko,
Thanks for your valid point. I should consider that point as well.
Regards,
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