Venkata Harsha Vardhan Boddeda

4 Questions 12 Answers 0 Followers

Questions related from Venkata Harsha Vardhan Boddeda

Can we use Polyethyleneimine siRNA for in vivo transfection?

Hi, I would like to deliver siRNA intrathecally in rodents. Can we use PEI as a vector for siRNA to deliver? Or do we have any other alternate vectors available? Thank you, Harsha

01 January 2019 1,708 0 View

Can we do Western Blotting even though the samples are kept in trizol?

Hi, I have mistakenly added tissues into the Trizol reagent and snap froze it. Later I realized I did a mistake and immediately removed the tissues out of Trizol and rinsed with PBS. Does it...

10 October 2018 1,777 4 View

Can we pool the blood from various mice for PBMC collection?

I would like to isolate PBMCs from mice. Is it possible to pool the blood from different animals? I would like to know if there would be any incompatibility between animals? Thank you Harsha

05 May 2018 5,731 0 View

Does a mouse primary antibody work in a rat tissue for IHC purpose?

I have a mouse primary antibody and I would like to use the same in the rat tissue for IHC purpose. Does that really work? Thank you Harsha

09 September 2017 8,401 4 View