Hi,
I would like to deliver siRNA intrathecally in rodents. Can we use PEI as a vector for siRNA to deliver? Or do we have any other alternate vectors available?
Thank you,
Harsha
Hi, I have mistakenly added tissues into the Trizol reagent and snap froze it. Later I realized I did a mistake and immediately removed the tissues out of Trizol and rinsed with PBS. Does it...
09 October 2018 1,692 4 View
I would like to isolate PBMCs from mice. Is it possible to pool the blood from different animals? I would like to know if there would be any incompatibility between animals? Thank you Harsha
04 May 2018 5,650 0 View
I have a mouse primary antibody and I would like to use the same in the rat tissue for IHC purpose. Does that really work? Thank you Harsha
08 September 2017 8,297 4 View
Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...
03 March 2021 401 4 View
How can we calculate the sample size for rodents for a study for one year? Are there any specific protocols to be followed for designing rodent sampling? How long should be the sampling interval...
01 March 2021 1,056 3 View
Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...
01 March 2021 2,512 1 View
Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...
28 February 2021 4,868 2 View
Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...
24 February 2021 1,825 3 View
Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...
22 February 2021 592 1 View
For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...
20 February 2021 4,332 4 View
1. A bcc metal single crystal was sliced along (1 -1 0) plane for TEM study. Assuming that the TEM available is equipped with a goniometer stage which tilts up to 45o. The Burgers vector of...
20 February 2021 2,370 1 View
i have a vector 1*512 size, i want to take the first value every 8 elements and put these elements at the end of the vector,how to do this on matlab ?
19 February 2021 2,352 5 View
Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...
15 February 2021 4,353 3 View