I got a delivery of electrocompetent and chemically competent cells from NEB (both derivatives from DH10 beta cell). The package says they have to be stored at -80 degrees, but the person who received the package stored them in -20 freezer instead and I didn't realise about it for 2 days. Is the transformation efficiency of the cells going to be deteriorated in any way? I want to use them with a very sensitive sample. I don't have that much DNA for my assembly (which I'm going to transform into these cells), so I don't have that much sample to test the competency of the cells.
Thank you very much in advance for your help.
Hi there,
There shouldn't be any problem provided the samples remained frozen at any time. The temperature of storage influences the stability of the competency on the long term issue.
Hi, Dominique. Thank you very much for your reply. I'll use them with more confidence, then.
Should be fine. Perhaps they will growth slightly slower (which I dont believe...).
Thanks for your comments, Dario. I'll try them first using the low copy number plasmid without the insert (before the assembly) and see how they work and how efficient are during the transformation.
I have tested the cells and they are working very well, with high efficiency. So it definitively doesn't affect their competency.
Thank you all for your comments.
- Badges
- Science topic