do you think we can trap lymphocytes to manage Lupus crisis?
Something like put a generator of a olecul which attract its and after take of the generator.
By this way we could down lymphocyts number without use immunosuressive drugs, no?
Dear Pierre
I am not very clear what you are trying to achieve. Do you want to deplete lymphocytes by non-immunosuppressive and non-cytotoxic means? Are you suggesting a physical method like leucopheresis? Theoretically possible but first you need to know which type of lymphocytes (B, T, Th1, Th2, Treg… ) you want to deplete. Then you could use an antibody against a lineage specific antigen. Which lymphocyte subset you are interested in?
Good luck.
Siva
Thank you for this answer. Now I know it exist different ways to take of some lymphocytes. The problem with Lupus, the imunitary system attack the body. It create many inflammation zones which can be easily identified. In the best case, I would like to find a way to trap what is the most specific to this pathogen inflammation zones without now what it is exactly. The pathogen inflammations appears by periods. That s why I am curious about if it is possible to set up a trap during a pathogen inflammation to attract the pathogen lymphocyte and take off its. The aim would be to down its numbers without use a drug which act on the cells multiplication (like it is done actually in France)
I think it is hard to conceive a strategy of trapping lymphocytes as a whole as a reasonable measure to fend a lupus crisis. There are regulatory lymphocytes, both T and B lymphocytes, that are already working to do that and they could be trapped as well. Further, under the systemic inflammatory milieu of a lupus crisis there are also memory effector lymphocytes that are needed for defense purposes and checking them we would compromise the patient's best outcome. Trapping sounds good, but we still need to do better in identifying and getting just the bad guys.
the "bad guy" and "good guy" have at least one difference that the "bad guy" attack the body in easy to identify zones. Do you think we could identify its by comparing the blood population of a lupus inflammation and a classic inflammation? Then try to delete its by the "antibody against a lineage specific antigen" method proposed by Muttuswamy Sivakumaran ?
I think it may be more complex than that. First, we can think about the autoimmune T cell clones that are probably expanded during a lupus flare. They could be identified by their idiotypic earmarks or some other more recent genetic biomarker, and then selected them out by trapping (this is because we started this conversation talking about trapping, whatever that trapping method may be). This cell selecting out could be approached systemically or even a the target organ. That would subsequently ameliorate the expanded systemic inflammatory response brought about by the paracrine effect of cytokines and chemokines on by-stander and locally attracted innate immune cells.
Very well, so it is complex but possible and it would be interesting to start by autoimmune T cell. I want to try to identify this autoimmune T cell and after we could try to imagine ways to capture and select the best. Do you have in more detail a specific way to identify this autoimmune T cell, can you advise me a article, study or publication? Else do you think I could do the difference after centrifugation to select lymphocyte, then use detergent to delete membranes and then compare electrophoresis results of a healthy and autoimmune place?
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