Dear all,
I am trying to determine the molar absorbance coefficient of specific Vitamin E metabolites (e.g., α-13'-COOH) and aim to measure their concentration at 292 nm. I would appreciate it if anyone could provide insights into this.
Dr Balamurugan Krishnamoorthy
Create a Calibration Curve: If available, use α-13'-COOH to prepare standard solutions and measure absorbance at 292 nm to establish its specific molar absorptivity. Use Similar Compounds: If α-13'-COOH is unavailable, approximate its coefficient using closely related Vitamin E metabolites like α-tocopherol.
The molar absorptivity of α-13'-COOH at 292 nm isn’t widely documented, but similar Vitamin E metabolites generally exhibit values around 2,500–3,000 L·mol⁻¹·cm⁻¹ at this wavelength. To estimate it accurately:
This approach can help you estimate the molar absorptivity for concentration calculations.
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William Letter
I would expect it to be the same for the Carbon-13 version as the regular version. C13 is not known to 'add' anything to the spectra (I think that is why there is little data on it)
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