I want to do a binding assay between the protein and its receptor. So, the protein that I will inmobilize to the CM5 chip will be the receptor that is specific for my ligand of interest. The ligand is a recombinant protein that will be a drug, so in all of cases, the pharmaceutics use BSA like a stabilizer, so they add in the excipient. I think that BSA can not bind to the receptor because the receptor is specific to one protein, but BSA can bind to the dextran matrix of the chip, so I thought to inmobilize the receptor on the ligand channel and inmobilize BSA on the reference channel at the same RU, then I was thinking to block with ethanolamine using amine coupling method. Thus, when I will pass the fluid with my protein of interest that also contains BSA, only the protein of interest binds to its receptor and probably BSA wont bind to the dextran matrix because there wont be more free sites.

Do you think my idea is OK? Please let me know your opinions. It will be helpful

Thanks everyone!

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