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Questions related from Jeremy Pronchik
Any dye recommendations or resources that tabulate more than Ex/Em wavelengths are appreciated. I am moving away from rhodamine and looking for a simpler fluorophore. Rhodamine is too...
12 May 2025 5,107 2 View
Using a fluorophore rather than a chromophore for ELISA detection seems like an obvious solution to increasing ELISA sensitivity, but I'm struggling to find work done in this area. Can anyone...
08 August 2019 2,870 5 View
free as in unreacted sulfydryls, not free as in speech, nor free as in beer :) I am working with an 80 kDa plasma protein with 5 cysteines, 2 of which are in a disulfide bridge. There are a lot...
15 December 2017 8,051 13 View
I would like to compare the polystyrene plates we use for ELISA assays to glass. Does anyone have experience either passively coating or covalently linking a capture antibody to a glass surface? I...
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