When I dilute Vemurafenib (initially in DMSO) in methylcellulose or PBS, it forms a block of precipitate that I can't dissolve, even by warming the solution.
You could try adding 5% PEG in Water to dilute the DMSO stock.
Hi, can someone please help me with their expertise. To analyse a dataset with 2 IV´s (2 levels each) and 1 DV (time - measured at 4 points, i. e. 4 DV?) I found the two-way repeated measures...
24 July 2024 3,284 6 View
I have synthesized MOFs already. However, I need to scale it up to produce 100g of MOFs. During my calculation, I need 15L of DMF just for this synthesis! So I thought, maybe DMF is just used as a...
01 July 2024 1,905 1 View
We have bumped into this energy drink, Celsius, with 1000% of RDA. I am starting to collect some information about this, but there is paucity of information.
08 June 2024 4,733 0 View
I have synthesized MOFs and been washing for about 2 weeks now until I reach the goal of UV absorbance of near 0.
27 May 2024 2,295 0 View
We want to keep AML cell lines together with T cells in serum free medium for about 4-7 days. Currently we culture them in RPMI 1640 with 10% FCS, so something resembling that would be good I...
22 May 2024 1,763 0 View
I usually wash it with MeOH by stirring (10 min.) then centrifuge at 8000rpm (10 min.) followed by DI water washing by stirring (10 min.) and then centrifuge again. I do it for 3x. The final one...
12 May 2024 8,835 0 View
I am currently working on my Thesis proposal and I am looking for the best way to approach MA or PhD professionals in the field of Special Education. To be specific, I will determine level of...
06 May 2024 5,879 4 View
Need advice on user-friendly tools to ease meta-analysis process of selecting eligible and fugitive studies
02 May 2024 2,332 3 View
Hi all, I have a set of cell culture samples embedded in 1% agarose. They have been cultured for 14 days. They have been fixed with 4% PFA. I am wondering if I can separate the whole cells from...
26 April 2024 6,030 1 View
I am writing my research using the GT of Strauss and Corbin. However, typical research templates always have this Conceptual or Theoretical Frameworks.
09 April 2024 3,076 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View