Could expired BSA a problem for tissue culture?

Hi, I want to ask if for you using expired BSA powder (expired in 2014 but always in the fridge and protected from light) could be a problem for my bovine primary follicles culture in situ (so...

01 December 2020 8,650 2 View

Neyman - Scott Cluster Process: are these nonsensical parameter values ?

I am trying to fit a cluster model in spatstat in R using the locations of archaeological sites. The resulting parameters values are: trend: 2.514429e-06 kappa: 1.606992e-08 scale: 481.5851 mu:...

18 November 2020 2,993 3 View

Flow cytometry from harvested non-uniform tissue?

Hi everyone! I want to identify by flow cytometry the immune cells found in the brain with a localized injury. My hypotesis is that the injury will attract more/different immune cells respect to...

27 October 2020 748 2 View

What do you use to avoid insect collection infestation?

At the museum we are currently dismissing Paradichlorobenzene and Naphtalene. we are looking for other effective chemical or similar.

21 October 2020 9,553 11 View

Issue with plasmid cloning?

22 August 2020 1,687 15 View

Is there any commercial or open source DIC (2D or 3D) software which is capable of working on field photographs or satellite images?

Is there any commercial or open-source DIC (2D or 3D) software which is capable of working on field photographs of terrain or satellite images for deformation and strain analysis in geophysical...

11 August 2020 4,216 3 View

How to perform in silico peptide cyclization reactions by ChemAxon Reactor?

24 April 2020 9,398 1 View

Does the EGFP epression in a BAC-EGFP mouse correlate with the amount of promoter gene expression?

Hi, I am analyzing immunostainings of 5-HT2AR-EGFP transgenic mice (Htr2a Gene and 5-HT2A Receptor Expression in the Cerebral Co... ) and I have a doubt regarding the conclusion I can draw about...

21 July 2019 209 1 View

Is it possible to "paste" two functions in COMSOL?

Hi everyone, here is the description of the problem. I have a rectangle with some circular holes in it, the inner part of the domain (denoted with D) is defined by the difference between the...

07 July 2019 1,635 3 View

How to avoid for order effects in an experiment with 20 participants and one task with 12 trials?

Hey, we are studying the relationship between trait anxiety and spatial navigation. We have 20 participants and one navigation task with 12 trials. How to avoid for order effects among the trials?...

07 April 2019 9,479 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

How do you mesh a thin solid in Fluent?

Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...

24 February 2021 8,856 3 View

Inpatient DBT to reduce parasuicidal behaviour?

Currently trying to obtain as many pieces of research as possible for my dissertation around the implementation of DBT within the inpatient unit for those with a diagnosis of BPD. Primarily...

23 February 2021 5,654 2 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View