Do you know how to obtain a HBEC-5i cell line monolayer?

25 November 2022 1 5K Report

Hello everyone! I am currently working with human endothelial cells from BBB (cell line HBEC-5i, ATCC) and I have a problem on obtaining a complete monolayer. The cells are harvest in DMEM:F12 media containing 10% FBS and 40 ug/mL Endothelial Cell Growth Factor (ECGF) according to the manufacturer indications. Also, I make 0.1% gelatin coating on everything (flasks, coverglass), but unfortunately, I did not manage to obtain monolayer, but rather small cell agglomerations. Do you have any suggestions?

Till Kasch

Hi Roberta, I've the same problem with HBEC-5i cells, have you already found a solution for the monolayer issue?

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