Explain plasmid description?

Hello everyone, I got a plasmid containing E. coli, and its description on the tube is Bacterial stab (DH5a)_HlZ31_cr_Amp_, I know that Bacterial stab refer to LB medium, DH5a is the name of E....

14 January 2021 6,326 2 View

Can I do gene expression with partial cds (not the complete one)?

Hello everyone, I got a partial cds, and I want to express it in yeast, does it work with partial cds or I need a complete one? If partial cds is possible, can you please suggest me some articles...

27 December 2020 3,688 3 View

How to calculate the Water Stress Index for a basin?

Water Stress Index (WSI) and Environmental water requirements (EWR). Thank you.

06 December 2020 904 3 View

What is a popular and standard model for quantifying land change drivers/determinants?

I'm looking for a standard model to quantify the land change drivers (e.g., forest to agriculture or vs). Actually, it depends on the data that I have and the specific location, but I would like...

22 November 2020 7,736 3 View

How can I discolor solution inside Ag/AgCl reference electrode?

Recently, I am using Ag/AgCl as RE in a basic three-electrode system to scan CV in K3Fe(CN)6/ K4Fe(CN)6. I realized that the color of the solution in RE changed from transparent to yellow. Can...

25 August 2020 6,254 7 View

I added Akaline Phosphatase and ligase buffer into my linearized plasmid without ligase but there are a lot of colonies after transformation.how come?

I am trying to find out what are the problems in my ligations. The first test: linearized Plasmid+ AP+ ligase buffer, after transformation, it gives a lot of colonies ! The second test:...

28 July 2019 8,550 3 View

How to combine fold change in microarray meta-analysis?

Hello, I would like to calculate the foldchange of differentially expressed genes after I meta-analyzed 9 datasets. I have calculated the fold change of those genes in each datasets but I wonder...

30 May 2019 459 3 View

Sample size for cluster analysis?

I have a follow-up question related to sample size for cluster analysis. Many recommend using 2^n - n is number of variables. I would like to ask if we should adjust for finite population...

30 January 2018 124 1 View

How could the morphology of anatase TiO2 (XRD) changed after grafting by amino propyl trimethoxy silane ? Thank you in advance.

I took XRD of TNTs and The modified TNT. And they were different?

14 February 2017 6,762 4 View

What is the effect of salt solution as the pore fluid on the pre-consolidation pressure of soils?

Dear all, I am doing oedometer test on remolded soils with sodium chloride (NaCl) as the pore fluid. The pre-consolidation pressure (Pc) was determined based on the method of Casagrande. The value...

13 December 2016 6,250 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View