How to clean up an already pure protein from grwoth medium?

Hi all, I have got a protein, expressed in P. pastoris vector, more than 97% (SDS-page) pure in the culture medium after cells separation. Despite the protein expressed has a his-tag, Nichel...

04 February 2021 5,173 6 View

What is your sentiment concern the impact of Covid19 on the main industries in Eu and USA ?

Covid 19 seem to be an huge crisis factor for the economy of several country . Im looking data for a first evaluation on EU and USA economy for to understand the best action for fight this impact .

07 April 2020 5,254 10 View

Direct viral RNA detection (RT_PCR)?

Is it possible, in your opinion, to detect RNA using RT-PCR but without RNA extraction? Considering for example a pharyngel swab sample, it is possbile to do a pre-treatment in order to preserve...

29 March 2020 7,576 5 View

Parameters selection for HDBSCAN algorithm in Supervised setting ?

I would like to know if there is any kind of research on the parameter selection (minpoints) for the HDBSCAN algorithm, in the context of having a training set with only normal samples and a test...

06 March 2020 3,487 3 View

How can I solve convergence problems of a dynamic analysis of an earth dam with opensees?

Hello everyone! I'm trying to model a earth dam with opensees. To do TCL file, I used GiD ProblemTypes for 2D Slope Analysis Input File Generation. When I do dynamic analysis with small...

03 September 2019 2,508 2 View

Are ACO and PSO ranking based feature selection approaches?

Hello, I've just came across these two algorithms and I was wondering whether there are any available versions for ACO and PSO as ranking-based feature selection approaches? Any comment would be...

18 July 2019 4,528 8 View

How can I calculate the aerodynamic characteristics of horizontal tail in FLUENT?

22 May 2019 6,252 3 View

How can I overcome the 230/260 low ratio problems after DNA clean up? and why do you think i got this problems?

Hi to everybody, I am doing the cloning experiment but I have problems before performing the digestion as my clean-up products have a 260/230 ratio of 0.5. There is someone available to explain...

03 May 2019 5,354 8 View

Where can I find the right CHARMM22 parameters for my paramagnetic complexes?

Hello, I'm trying to model some gadolinium complexes through molecular dynamics using the parametrized CHARMM22 force field as described in International Journal of Quantum Chemistry, Vol. 73,...

04 April 2019 6,889 1 View

How can Principal component analysis (or factor analysis) be applied on a questionnaire score, and how can the results be intepreted?

During my research, I asked several companies to complete a questionnaire to measure they risk maturity. At the end of the questionnaire, each respondent receives a score from 1 to 5 (1=low...

26 March 2017 491 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Is there an open source free software for text mining and sentiment analysis?

I am looking for a free software for text mining and sentiment analysis for my research on customer review mining (it involves calculating polarity of attributes,opinion oriented information...

25 February 2021 1,920 6 View

What software programs can directly simulate Additive Manufacturing Directed Energy Deposition (DED)?

I understand there are a lot of software programs that can simulate DED by going through specific modeling, simulation, and commands. For me, that was time consuming and results were not accurate....

24 February 2021 4,428 3 View