We have been using thinner to remove the insect specimens from sticky traps. However, we would like to use other solvents with no toxic effects.
Thank you very much for your quick answer! I also came across this paper, however, these solvents also seem to be toxic to humans.
Thank you very much!
Hi Negin!
We use Citrosol Cleaner (citrus oil, almost pure d-limonene) for this matter, it works very well, is non toxic (for humans) and has a pleasant citric smell :)
See you,
Rik
Hi Rik,
Many thanks!!! That would be great!
Do you have any experience with the quality of the DNA extracted from specimens treated with Citrosol Cleaner?
Hi Negin,
I asked my colleagues, the psyllid samples they used this for, for some reason never underwent further DNA extraction. So I can't say.
It seems, it could have an effect (or require additional washing steps) see link.
https://www.google.com/url?sa=t&source=web&rct=j&url=https://static.cdfa.ca.gov/PiercesDisease/proceedings/2008/2008_12-15.pdf&ved=2ahUKEwjQ2ID9-aPoAhVE-aQKHTZ9Dr0QFjABegQIAxAB&usg=AOvVaw3hnEmmLSS1Fu2THuKaC8Ds
Thanks Rik! I will give it a try. Hopefully, this oil will not interfere with our molecular analysis.
Use some 70% ethanol to remove the stickyness. Also 25% acetone in water is effective to remove the stickyness, if it does n't affect your samples.
Hi Justin,
Thanks for your answer! I used ethanol but it was not possible to remove the glue completely.
Acetone should work, and you can adjust the concentration based on the stickiness. We use it to remove insects from sticky cards.
following.
Thank you very much Justin!
The use of oil as Ricinus communist coul resolve your problem!!
I want to know more about Uranium ore deposits in world.
11 August 2024 6,720 0 View
I want to know more about diamond ore deposits in world.
11 August 2024 2,167 1 View
We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...
10 August 2024 6,678 14 View
If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?
10 August 2024 8,198 5 View
I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...
10 August 2024 3,229 0 View
What are a “Farmers Producer Organization” (FPO) and its essential features?
10 August 2024 477 5 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?
09 August 2024 3,291 2 View
I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...
08 August 2024 4,831 4 View
How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?
08 August 2024 8,162 0 View
I need help for find a subject for PhD in insect physiology
12 July 2024 8,227 1 View
I want to know what are the relationship that happen between insect biodiversity and durian tree
10 July 2024 8,230 1 View
My colleague and I started research project about bioplastic made out of starch-curcumin composite. However, we are currently having trouble regarding the produced film. We have degassed the...
01 July 2024 9,419 0 View
I need more information about this topic
27 June 2024 4,057 3 View
I am trying to estimate ROS in insect tissue. Is it possible to do it using DCFDA
18 June 2024 6,954 0 View
I'm a student of biological sciences and I need to collect information about how ambient temperature affects insects behaviour to answer the question: " which insects are more active in...
15 June 2024 367 3 View
I know that all animals and insects produces hormones. Do plants produce hormones that can have an equivalent effect on an animal like animal hormones?
13 June 2024 3,860 1 View
Aquatic insects
11 June 2024 8,144 3 View
Why Spine gourd fruit become red while it is tender ? No attack of insect pest is recorded.
11 June 2024 8,771 0 View
hello Can any one identification this insect best wishes
21 May 2024 837 8 View