I am analyzing some results and I find no increase in ROS production but cortical neurons die anyway. This is not the first time that our group struggles with these results. It is hard to explain cell toxicity without ROS production since it is the most "known" and accepted mechanism, at least in cultured neurons. So, now I am searching for literature with experiments that may have found a possible alternative pathway for cell death.
iT IS NOT IN NEURONS BUT IN ASTROCYTES:
Jazmin Gabriela Reyes Ocampo et al.(2015) Mitochondrial dysfunction related to cell damage induced by 3-HK and 3-HANA: not dependent-effect of reactive oxygen species production. Neurotoxicology. (ACCEPTED)
Cell death is not always associated with ROS production! Apoptosis is some times quite good and simple explanation.
Thanks Nikola! The thing is that in some of our previous work we have seen apoptosis after ROS production. However, in the present conditions, something else is happening... I could not yet find what is triggering apoptosis in these cells.
Well try determining some signal molecules caspases, FAS or something like that. Also cells can die due to necrosis.
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