molecular detection of chicken anemia virus live attenuated vaccine strain (lohmann & inter vet)
Dear Aalaa Samir,
In general, there is no significant difference in CAV genomes irrespective of geographic origin. Comparison of the complete or partial genome sequences available in GenBank resulted in very little difference among them, and the diversity of nucleotide sequences of CAV is usually around 5%. VP2 and VP3 are conservative among strains, while VP1 seems to be the most different of CAV strains.
As far as I know, the 2 vaccine strains of Lohmann and Intervet vaccine are Cux-1 and 26P4. These 2 strains are not much different in their nucleotide properties. Therefore, we can simply use any designed primers that amplify partially VP2, and/or VP3 to detect CAV.
This paper included the primers used to detect CAVs.
Archives of Virology. January 2006, Volume 151, Issue 1, pp 97-111
Molecular epidemiology of chicken anemia virus in Nigeria (Ducatez et al., 2006).
If use need a high sensitivity of the detection, you can use nested PCR or real-time PCR (The PrimerDesign™ Kit for Chicken anemia virus, Genesig).
Best,
Dear Aalaa,
Check the primers from "Cardona CJ, Oswald WB, Schat KA: Distribution of chicken anaemia virus in the reproductive tissues of specific-pathogen-free chickens. J Gen Virol 2000, 81:2067–2075." for CAV detection. These primers are broadly reactive and should be able to amplify any CAV strain. Indeed, CAV is not very variable.
I have used them successfully to amplifiy CAV strains from several countries. I have only modified the cycling profiles of the PCRs (see Snoeck et al., Epidemiology of CAV in Central African Republic and Cameroon. Virol J 2012). You can even sequence directly the PCR products obtained.
I hope that this helps.
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