To the best of my knowledge you can consult any Pharmacopeia if you want to determine the allicin content, with an extraction with water and HPLC ODS column with a precolumn. Mobile phase Water: Formic acid 1% (60:40) and internal standard (sorry, I have the thecnique only in Spanish). But if you want to determine the presence by TLC, consult Wagner Plant Drug Analysis. Hope helped you!
My colleague Dr. M.M. Gupta, Chief scientist at Central Institute of medicinal and Aromatic Plants, Lucknow, India has published a number of plants on analytical procedures of medicinal plants. You can view his complete publications on Google Scholar. If you contact him, I hope he will help you.
Good luck.
allicin is a sulphus compound that in obtained from garlic, You can test any method which can show presence of sulphur content you will get equal proportion of alicin. a fast and cheap method for assaying allicin, the active constituent of garlic extract,
based on UV spectrophotometry. Garlic cloves are extracted using water. The allicin
content of the garlic extracts can be analysed after passing the extract through SPE
cartridge and eluted with solvents of various polarities. The most polar solvent used
is water which elutes the allicin in 4 ml, while methanol and ethanol do not show this property. The absorbance of UV radiation at 240 nm and 254 nm wavelengths by the garlic fraction elute with water gave a ratio of A240 nm/ A254 nm H” 1.4 – 1.5, which is typical for allicin.
Dear Marcel
Please find in attachment some easy method for estimation of allicin from garlic.
Thanking you
http://www.ncbi.nlm.nih.gov/pubmed/14969516
http://www.ncbi.nlm.nih.gov/pubmed/17221429
Thank you very much, everyone really very kind and helpful. We need a particular method with a spectrophotometer.
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