Hello I'm trying to detect Gli1 with immunofluorescence study in 4% PFA pre-fixed mouse dorsal skin. So far I could not detect it. Do you know a good antibody for mouse Gli1?
I am also trying to figure out which Gli1 antibody is working, I have tried several but no positive signals were obtained, may I know how is your experience, do you have a good Gli1 antibody now? May you share it with me as well as the protocol? Many thanks.