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19 January 2021 7,478 3 View

Multiple sequence alignment - polymorphism for restriction digest?

Hi, After comparing multiple (2+) DNA sequences, I want to identify ONLY the restriction sites that changed due to the detected polymorphism. I tried several things by hand, but it's WAY too...

23 September 2020 6,289 4 View

Would you be interested in the special issue "High Temperature Statistical Thermodynamics of Molecules in Gases and Plasmas", Entropy IF=2.494?

https://www.mdpi.com/journal/entropy/special_issues/Gases_and_Plasmas

26 August 2020 9,474 4 View

Can I run a chi-square test when my data include one 'zero' value? Can I run a post hoc analysis after chi-squre when p<0.05?

I have one row of raw data 13, 0, 24, 26, 17 and 14. It is a larval survival rate / six treatments. Can I run chi-squared with zero inluded? I use R, and it looks like it works, but I am not...

24 July 2020 7,720 5 View

How to identify crystals in the shrink cavity?

I have a problem. I watched the crystals on the surface of the shrinking cavity. How to recognize these elements? EDS analysis is not possible due to the depth of the shrink cavity. Machining can...

29 May 2020 4,235 0 View

How to choose the right protein subunit for symmetrical docking?

Recently, I have been dealing with symmetrical docking of protein complexes and I have a problem. Well, I have no problems with docking complexes with cyclic symmetry, it is double-walled symmetry...

09 May 2020 4,424 3 View

How to model a material in Abaqus with 2 types of behavior: high stiffness in compression and low stiffness in tension?

I am modeling a dynamic problem in Abaqus. The material should have two types of properties: different Young's moduli in tension and compression. Any simple way to achieve it? Thanks!

17 February 2020 8,563 2 View

How many water molecules are incorporated into the structure of fructan?

I am looking for help to calculate how many water molecules are incorporated into the structure of fructan. We want to apply polymer density functional theory (DFT) for prediction and description...

01 April 2019 7,096 3 View

No title

I test preference and survival rate of one population (moth larvae). I have six varieties of plants (a-f) in six replicates (36 in total) arranged in Latin square block. I applied 5 larvae (1st...

04 March 2019 5,183 3 View

How to understand "no change in luminescence band" when I can see this change?

26 June 2017 5,786 3 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

How could I process data in RCT to include it in meta-analysis?

I'm in the process of doing a meta-analysis and have encountered some problems with the RCT data. One of my outcom is muscle strength. In one study, I have three different measurements of muscle...

25 February 2021 7,603 3 View

Satellite orbit estimation using Recurrent Neural Network - what should I use?

Hello, I am a master's student studying in Yonsei Univeristy, Korea. I am trying to estimate the state of satellite, using Neural Network. Below is a simple flow of my study. 1. Train (t0 ~ t1)...

23 February 2021 6,383 3 View

Any Differences in COVID-19 vaccines?

Are there any differences (outside of the clinical trials that led to their EUA) observed in the rate of infection among those who have received the Pfizer vs Moderna vaccine since the mass...

22 February 2021 9,727 6 View

How can I get the coding gene sequences of Hela and U2OS cell lines? Is mRNA sequence of Hsp90 alpha tissue specific??

I am looking for hsp90 mRNA sequence in Hela and U2OS cell lines, I have tried through ncbi but always the output is hsp90 in homo sapiens not in the specific cell line. Kindly, could any one...

22 February 2021 1,853 3 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View

Difficulty in debugging code to correct error - TypeError: '<' not supported ?

I am trying with a Unet code ontained from net ...in tensorflow. When trying to predict the segmentation result with the trained and saved model with metric " iou" using "CustomObjectScope"...

22 February 2021 5,254 1 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View