04 August 2022 1 9K Report

I need to know this information in order to develop an ecotoxicology bioassay, but I have not find the exact data.

More Irina Patricia Tirado-Ballestas's questions See All
How to properly extract proteins from a Caco-2 cell line?

Hi everyone! As I previously shared with you, I've started to work with Caco-2 cells and even asked for your help. I recently followed a protocol to extract proteins, but when I collected the...

12 May 2024 6,819 2 View

How to prevent detection HA-tagged protein after IP if I look for other proteins?

Hello, I do IP using HA-tagged beads. One of my proteins through which I precipitate complex has HA-tag. I see this protein (with HA-tag) after IP. The signal is strong and bright. The complex...

28 March 2024 4,493 2 View

Can I simultaneously measure concentration of cytokines in serum and culture medium with Multiplex Luminex assay?

Dear colleagues, We are planning to use Human XL Cytokine Base kit for evaluation of concentration of several cytokines. The plan was to measure concentration of cytokines both in blood serum, and...

20 February 2024 7,692 0 View

What is the price (average) in USD of Microwave-assisted hydrodistillator with a 1000 mL flask?

I need to buy this equipment to obtain several essential oils.

18 February 2024 401 0 View

Can I perform re-extraction of RNA from eluted RNA (Column filter extraction protocol)?

I encounter the following problem: I want to send viral RNA for further analysis. As the receiver requires, I must process my samples according to a regular RNA extraction protocol and send the...

06 February 2024 7,465 4 View

Is this normal on my Caco-2 culture?

hi again! I had never worked with Caco-2 cells before and just recently I thaw some. When I look at them I see something that I really don't know what it is. I know these cells secrete a lot of...

14 January 2024 9,068 4 View

Development of a smart refrigerator for 1C?

What are the main advantages and potential applications of a smart refrigerator developed on the 1C platform, and what functionality can be implemented using this platform?

29 November 2023 8,294 1 View

why won't my IEC-6 cell culture grow?

hi. I'm having a hard time with my IEC-6 cell culture. I've been working with this cell line for a time now and never had any problems until now; there's cell detachment, weird morphology and they...

01 October 2023 9,832 2 View

Would anyone suggest me a good anti-Piezo1 antibody to use on IF-FPPF samples?

Hi! I am trying to detect Piezo1 on mouse kidney samples by IF (PFFP), but the antibody I am using is not the best: Proteintech #15939-1-AP. There are many different options to get a new one, but...

19 June 2023 148 0 View

Can I use a hot-start polymerase (antibody blocked polymerase activity) for amplification of PCR products before sequencing?

I have the following question: I have a nested PCR protocol that uses a chemically modified hot-start polymerase. However, in the lab, I have a hot-start polymerase, but bounded to an antibody....

16 May 2023 6,906 2 View

Similar questions and discussions
How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to assess the osmotic power of a substance?

Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...

06 August 2024 977 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

Assistance with MTT Assay Data ?

I did an MTT assay with 2 drugs (Piroxicam and Sulfameter) when I got my results for the 12,24,48,72 hours of incubation with my drug at the following concentrations [ug/ml]...

26 July 2024 7,511 3 View

Can I use sonicator only to lyse the exosomes and measure their protein contents using BCA assay kit?

Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...

23 July 2024 4,603 4 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

My question concerns MTT cell viability assay?

Despite not having cells in the media, I am getting purple color. I have tried many troubleshooting methods, varying media types, and even different MTTs from different companies to figure out the...

21 July 2024 9,914 1 View

How I can add anew research in my account ?

Their is new research published in the biological control journal, and I am one of the authors. I want to add it to my account at researchgare. How can I do this?

21 July 2024 7,545 0 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close