What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Special issue on PLS-SEM in Mathematics. Are you interested?

21 February 2021 5,601 3 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View

Is there any solar electric power plant having the concentrated photovoltaic modules?

The solar photovoltaic (PV) power plant uses commercial (non-concentrated) PV modules which are quite simple in design and reliable. They can work on fixed orientation and don't need the moving...

16 February 2021 3,796 9 View

Which drug is better to modify blood pressure in awake monkey, if I provide it in the food or drink reward?

I need to modify the blood pressure in a monkey while is awake in the recording chamber, and I am planing on provide a drug for that in the food or water used for reward... any advise? Thank you

08 February 2021 9,900 1 View

Why stop the reaction in ice for the fluorescent probe FDA (fluorescein diacetate)?

Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...

08 February 2021 3,366 3 View

How to make Fast Green FCF from powder?

Hi everyone We need Fast Green to electroporate plasmid into mice embryos, therefore we simply need it to see some color in the liquid we are electroporating so that electroporation is more...

07 February 2021 1,671 2 View

Why do high doses of humic substances inhibit the growth of corn roots? Is there a toxicity effect?

Length, volume, surface area and dry mass of corn roots treated with humic substances increased up to 13 liters per hectare and decreased with higher doses.

02 February 2021 2,190 4 View

Using a nucleosil c18 column, i get no negative peaks in real time and two negative peaks in post run.Software problem?

Simadzu, lc solutions

02 February 2021 1,969 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Macrophages in culture not growing/ dying or detaching . Why ?

Hello, I am an undergraduate student doing my dissertation, where I isolate PBMCs from leucocyte cones in order to plate monocytes and allow for macrophage differentiation. Although the numbers...

09 February 2021 5,334 8 View

Is there a reason why my monocyte population had high CD3 expression?

We ran a flow cytometry experiment where Fc blockers were added into the antibody cocktail. Other antibodies added include: 1. anti-CD3-PE-Cy7 2. anti-CD4-APC 3. anti-CD8-AmCyan 4....

10 January 2021 2,806 4 View

Is it possible to grow human monocytes in DMEM?

I need to purify monocytes isolated from human peripheral blood using the plate adhesion methodology. However, I only have DMEM in the laboratory. I did a pilot and the cells did not adhere...

09 December 2020 2,555 3 View

Do monocytes from mouse blood adhere, while T cells go into suspension?

I would like to know if mouse blood cells exhibit the same separation patterns as human peripheral blood. With human blood, it is possible to plate the white blood cells (after using a density...

15 November 2020 9,408 1 View

What is the mouse monocyte gating strategy?

I Traditionnaly work with germinal cells or fibroblasts but I have blood and bronchoalveolar lavage from mice that we would like to analyse on flow cytometry (monocyte population). However, the...

28 October 2020 9,191 2 View

What are the possible Antigen presenting cell in PBMC?

Hi everyone, I would like to ask, in PBMC when pulsed by peptide, what are the cells that present the peptide to the T cells? Is it B cell or monocyte? And is it memory T cells that are activated...

27 October 2020 7,018 3 View

Isolation of Mouse Monocytes for ADCC?

Hi All, Immunology novice here, looking for some advice on mouse monocytes. We are looking to isolate mouse monocytes suitable to demonstrate In vitro ADCC. we tried out two methods: Mothod 1:...

17 September 2020 2,076 3 View

Monocytes differentiation to M1 and M2 phenotypes Challenge. what went wrong?

I tried to differentiate monocytes toward M1 and M2 phenotypes. The method I followed: Firstly, I isolated monocytes from blood cones using Pan Mono isolation kit, human (MACS), Miltenyi. Then, I...

08 September 2020 4,150 2 View