María Teresa Herrera

4 Questions 26 Answers 0 Followers

Questions related from María Teresa Herrera

Are there any expert in dendritics cells derivated from somebody blood monocytes?

I am interested in the study of dendritic cells response under infection with mutant strain candidate to vaccine against TB. But I am begginer in the work with dendritic cells, somebody can help...

21 March 2024 1,550 7 View

Can you recommend any tip for to take off mdm fro low adhirience culture plate?

Mi objetive is to sort MDM infected from uninfected. MN were purify from PBMC using Miltenyi sytem (CD14). MDM are obtained after 7 days of monocytes culture in low adherence culture plate. MDM...

23 June 2018 9,993 9 View

Do you have an alternative method for RNA re-purification different to sodium acetate precipitation?

I purified the RNA from whole blood by columna (Paxgene kit), and the RNA has 260/280 ratio 2.0, but the ratio 260/230 is less than 0.94. The purificación methodology includs DNAse diggestion In...

31 July 2015 3,729 10 View

Do you have CD14 negative population after you purify monocytes?

I purity MN by positive selection using Miltenyi technology (anti-CD14), and these MN are used for M.tuberculosis-DAF phagocytosis assay. MN are stained with Ab anti-CD14-PE-Texas red. The...

16 June 2015 3,200 5 View