Hi every one. I have to cut some paraffin embedded lung tissues in serial sections with 1 micrometer. The tissue gets shredded as I switch from 2-3 um to 1 um.
Thanks for your help.
https://www.jove.com/t/58288/the-cutting-floating-method-for-paraffin-embedded-tissue-for
I would not recommend to cut 1µm thick paraffin scetions. The paraffin block is to soft to cut in these ranges. For cutting 1 µm or thinner you need epoxid or plastic like metharylate resins because thesen resins are suitable for thicknes below 1µm. Epoxide or Araldit are the embedding resins for electron mircoscopy (thicknes 100nm or thinner).
I would recommand to cut 2-3 or even to 5µm (the difference is not so big). Try to prepare section ribbons of 10 to 15 sections and collect them in paper boxis. After you have finished sectioning you start mounting your sections. For example take one section of each ribbon and mount it on the slide. You can divide the ribbons with the help of razor blades or scapell blades. to stretch the sections you can use either a water bath or you put a drop of water on the slide and dry it out on a heating plate.
Afterwards you can use any stain or immunohistostochemisty procedure.