I'm starting working with SW48 colon cancer cells in vivo, and I wondered if Matrigel is needed for a subcutaneous injection. And, how much cells do you generally inject?
I had also worked on B16F10 melanoma cancer cell line in C57BL mice. So i had Injected 5 Lakh cell/ 200ul with the help of 1ml syringe.I had counted cell in heamtocytometer dye with typhen blue dye.May be its helpful for you.